Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks

Huang Liu; Yi Chen

doi:doi:10.11648/j.sd.20190703.13

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Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks

Huang Liu, Yi Chen

Published in Science Discovery (Volume 7, Issue 3)

Received: 19 April 2019 Published: 15 June 2019

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Abstract

In this study, a simple yet effective protein detection method was established by using bimetallic Au@Ag core-shell nanobrick as SERS active building blocks. The Au@Ag core-shell nanobrick is synthesized by well-defined ‘two-step’ growing method, enabling uniform and controllable silver shell growth on the surface of the gold nanorods induced by surfactant replacement. The thickness of the silver shell can be programmably adjusted by controlling the amount of silver nitrate in the system, which in turn realized the controllable adjustment of the optical properties of Au@Ag core-shell nanobrick. We selected bovine serum albumin as a model protein and established a highly-sensitive assay for label-free SERS detection of protein. The detection limit can reach to as low as 0.5 μg/mL, enabling promising avenue for its clinical application in ultrasensitive biomarker detection.

Published in	Science Discovery (Volume 7, Issue 3)
DOI	10.11648/j.sd.20190703.13
Page(s)	147-151
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Plasmonic Nanoparticle, Au@Ag Core-shell Nanobrick, SERS, Label-Free Protein Detection

References

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APA Style

Huang Liu, Yi Chen. (2019). Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks. Science Discovery, 7(3), 147-151. https://doi.org/10.11648/j.sd.20190703.13

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ACS Style

Huang Liu; Yi Chen. Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks. Sci. Discov. 2019, 7(3), 147-151. doi: 10.11648/j.sd.20190703.13

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AMA Style

Huang Liu, Yi Chen. Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks. Sci Discov. 2019;7(3):147-151. doi: 10.11648/j.sd.20190703.13

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@article{10.11648/j.sd.20190703.13,
  author = {Huang Liu and Yi Chen},
  title = {Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks},
  journal = {Science Discovery},
  volume = {7},
  number = {3},
  pages = {147-151},
  doi = {10.11648/j.sd.20190703.13},
  url = {https://doi.org/10.11648/j.sd.20190703.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20190703.13},
  abstract = {In this study, a simple yet effective protein detection method was established by using bimetallic Au@Ag core-shell nanobrick as SERS active building blocks. The Au@Ag core-shell nanobrick is synthesized by well-defined ‘two-step’ growing method, enabling uniform and controllable silver shell growth on the surface of the gold nanorods induced by surfactant replacement. The thickness of the silver shell can be programmably adjusted by controlling the amount of silver nitrate in the system, which in turn realized the controllable adjustment of the optical properties of Au@Ag core-shell nanobrick. We selected bovine serum albumin as a model protein and established a highly-sensitive assay for label-free SERS detection of protein. The detection limit can reach to as low as 0.5 μg/mL, enabling promising avenue for its clinical application in ultrasensitive biomarker detection.},
 year = {2019}
}

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TY  - JOUR
T1  - Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks
AU  - Huang Liu
AU  - Yi Chen
Y1  - 2019/06/15
PY  - 2019
N1  - https://doi.org/10.11648/j.sd.20190703.13
DO  - 10.11648/j.sd.20190703.13
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 147
EP  - 151
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20190703.13
AB  - In this study, a simple yet effective protein detection method was established by using bimetallic Au@Ag core-shell nanobrick as SERS active building blocks. The Au@Ag core-shell nanobrick is synthesized by well-defined ‘two-step’ growing method, enabling uniform and controllable silver shell growth on the surface of the gold nanorods induced by surfactant replacement. The thickness of the silver shell can be programmably adjusted by controlling the amount of silver nitrate in the system, which in turn realized the controllable adjustment of the optical properties of Au@Ag core-shell nanobrick. We selected bovine serum albumin as a model protein and established a highly-sensitive assay for label-free SERS detection of protein. The detection limit can reach to as low as 0.5 μg/mL, enabling promising avenue for its clinical application in ultrasensitive biomarker detection.
VL  - 7
IS  - 3
ER  -

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Author Information

Huang Liu

State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science & Medical Engineering, Southeast University, Nanjing, China
Yi Chen

State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science & Medical Engineering, Southeast University, Nanjing, China

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APA Style

Huang Liu, Yi Chen. (2019). Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks. Science Discovery, 7(3), 147-151. https://doi.org/10.11648/j.sd.20190703.13

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ACS Style

Huang Liu; Yi Chen. Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks. Sci. Discov. 2019, 7(3), 147-151. doi: 10.11648/j.sd.20190703.13

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AMA Style

Huang Liu, Yi Chen. Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks. Sci Discov. 2019;7(3):147-151. doi: 10.11648/j.sd.20190703.13

Copy | Download

@article{10.11648/j.sd.20190703.13,
  author = {Huang Liu and Yi Chen},
  title = {Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks},
  journal = {Science Discovery},
  volume = {7},
  number = {3},
  pages = {147-151},
  doi = {10.11648/j.sd.20190703.13},
  url = {https://doi.org/10.11648/j.sd.20190703.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20190703.13},
  abstract = {In this study, a simple yet effective protein detection method was established by using bimetallic Au@Ag core-shell nanobrick as SERS active building blocks. The Au@Ag core-shell nanobrick is synthesized by well-defined ‘two-step’ growing method, enabling uniform and controllable silver shell growth on the surface of the gold nanorods induced by surfactant replacement. The thickness of the silver shell can be programmably adjusted by controlling the amount of silver nitrate in the system, which in turn realized the controllable adjustment of the optical properties of Au@Ag core-shell nanobrick. We selected bovine serum albumin as a model protein and established a highly-sensitive assay for label-free SERS detection of protein. The detection limit can reach to as low as 0.5 μg/mL, enabling promising avenue for its clinical application in ultrasensitive biomarker detection.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks
AU  - Huang Liu
AU  - Yi Chen
Y1  - 2019/06/15
PY  - 2019
N1  - https://doi.org/10.11648/j.sd.20190703.13
DO  - 10.11648/j.sd.20190703.13
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 147
EP  - 151
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20190703.13
AB  - In this study, a simple yet effective protein detection method was established by using bimetallic Au@Ag core-shell nanobrick as SERS active building blocks. The Au@Ag core-shell nanobrick is synthesized by well-defined ‘two-step’ growing method, enabling uniform and controllable silver shell growth on the surface of the gold nanorods induced by surfactant replacement. The thickness of the silver shell can be programmably adjusted by controlling the amount of silver nitrate in the system, which in turn realized the controllable adjustment of the optical properties of Au@Ag core-shell nanobrick. We selected bovine serum albumin as a model protein and established a highly-sensitive assay for label-free SERS detection of protein. The detection limit can reach to as low as 0.5 μg/mL, enabling promising avenue for its clinical application in ultrasensitive biomarker detection.
VL  - 7
IS  - 3
ER  -

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